Opportunities and challenges of liquid chromatography coupled to supercritical fluid chromatography

The analysis of complex samples is facilitated by the emergence two-dimensional liquid chromatography. Despite optimization efforts to reach tremendous peak capacities, separation neutral compounds remains limited. Indeed, most combinations chromatographic modes suffer either from a lack orthogonality or serious solvent incompatibilities. involving combination chromatography and supercritical fluid seems offer new opportunities for ionizable solutes. This review highlights can thanks wide range stationary phases. Injection effects occurring in SFC, that may drastically reduce performances online LC × SFC are also discussed details. Finally, despite net improvement instrumentation recent years, instrumental limitations still have be overcome. While this potential its complementarity with 2D-LC, it demonstrates development requires joint researchers suppliers before comprehensive gets adopted scientific community. • molecules limited LC. Two-dimensional exhibit interesting molecules. under investigation. Specific interfaces been proposed but commercial lacking.